T-cell Responses to Individualized Neoantigen Therapy mRNA-4157 (V940) Alone or in Combination with Pembrolizumab in the Phase 1 KEYNOTE-603 Study.
Gainor Justin F, Patel Manish R, Weber Jeffrey S, Gutierrez Martin, Bauman Julie E, Clarke Jeffrey M, Julian Ricklie, Scott Aaron J, Geiger Jessica L, Kirtane Kedar, Robert-Tissot Celine, Coder Brandon, Tasneem Moomal, Sun Jing, Zheng Wei, Gerbereux Lauren, Laino Andressa, Porichis Filippos, Pollard Jack Russella, Hou Peijie, Sehgal Vasudha, Chen Xing, Morrissey Manju, Daghestani Hikmat N, Feldman Igor, Srinivasan Lakshmi, Frederick Joshua P, Brown Michelle, Aanur Praveen, Meehan Robert, Burris Howard A
Cancer discovery · 2024 · PMID 39115419 · 인용 105
mRNA-4157 (V940) is an individualized neoantigen therapy targeting up to 34 patient-specific tumor neoantigens to induce T-cell responses and potentiate antitumor activity. We report mechanistic insights into the immunogenicity of mRNA-4157 via characterization of T-cell responses to neoantigens from the first-in-human, phase 1, KEYNOTE-603 study (NCT03313778) in patients with resected non-small cell lung cancer (Part A: 1-mg mRNA-4157, n = 4) or resected cutaneous melanoma (Part D: 1-mg mRNA-4157 + 200-mg pembrolizumab, n = 12). Safety, tolerability, and immunogenicity were assessed.
All patients experienced ≥1 treatment-emergent adverse event; there were no grade 4/5 adverse events or dose-limiting toxicities. mRNA-4157 alone induced consistent de novo and strengthened preexisting T-cell responses to targeted neoantigens. Following combination therapy, sustained mRNA-4157-induced neoantigen-specific T-cell responses and expansion of cytotoxic CD8 and CD4 T cells were observed. These findings show the potential of a novel mRNA individualized neoantigen therapy approach in oncology.
Significance: The safety and immunogenicity results from this phase 1 study of mRNA-4157 as adjuvant monotherapy or combination therapy with pembrolizumab show generation of de novo and enhancement of existing neoantigen-specific T-cell responses and provide mechanistic proof of concept to support further development of mRNA-4157 for patients with resected solid tumors. See related commentary by Berraondo et al., p. 2021.